Westphal, Volker

[["dc.bibliographiccitation.artnumber","11354"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Oracz, Joanna"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Radzewicz, Czesław"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:41:07Z"],["dc.date.available","2018-01-17T13:41:07Z"],["dc.date.issued","2017-09-12"],["dc.description.abstract","In STED (stimulated emission depletion) nanoscopy, the resolution and signal are limited by the fluorophore de-excitation efficiency and photobleaching. Here, we investigated their dependence on the pulse duration and power of the applied STED light for the popular 750 nm wavelength. In experiments with red- and orange-emitting dyes, the pulse duration was varied from the sub-picosecond range up to continuous-wave conditions, with average powers up to 200 mW at 80 MHz repetition rate, i.e. peak powers up to 1 kW and pulse energies up to 2.5 nJ. We demonstrate the dependence of bleaching on pulse duration, which dictates the optimal parameters of how to deliver the photons required for transient fluorophore silencing. Measurements with the dye ATTO647N reveal that the bleaching of excited molecules scales with peak power with a single effective order ~1.4. This motivates peak power reduction while maintaining the number of STED-light photons, in line with the superior resolution commonly achieved for nanosecond STED pulses. Other dyes (ATTO590, STAR580, STAR635P) exhibit two distinctive bleaching regimes for constant pulse energy, one with strong dependence on peak power, one nearly independent. We interpret the results within a photobleaching model that guides quantitative predictions of resolution and bleaching."],["dc.identifier.doi","10.1038/s41598-017-09902-x"],["dc.identifier.pmid","28900102"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11728"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation.eissn","2045-2322"],["dc.title","Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","571"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","573"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Han, Kyu Young"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Reuss, Matthias"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:10Z"],["dc.date.available","2017-09-07T11:44:10Z"],["dc.date.issued","2011"],["dc.description.abstract","Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells."],["dc.identifier.doi","10.1038/NMETH.1624"],["dc.identifier.gro","3142707"],["dc.identifier.isi","000292194500020"],["dc.identifier.pmid","21642963"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/141"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Sharper low-power STED nanoscopy by time gating"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","143903"],["dc.bibliographiccitation.issue","14"],["dc.bibliographiccitation.journal","Physical Review Letters"],["dc.bibliographiccitation.volume","94"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:54:29Z"],["dc.date.available","2017-09-07T11:54:29Z"],["dc.date.issued","2005"],["dc.description.abstract","Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down to 16 nm in the focal plane, corresponding to about 1/50 of the employed wavelength. The optical bandwidth expansion by nearly an order of magnitude is realized by a saturated depletion through stimulated emission of the molecular fluorescent state. We demonstrate that en route to the molecular scale, the resolving power increases with the square root of the saturation level, which constitutes a new law regarding the resolution of an emerging class of far-field light microscopes that are not limited by diffraction."],["dc.identifier.doi","10.1103/PhysRevLett.94.143903"],["dc.identifier.gro","3143865"],["dc.identifier.isi","000228390600015"],["dc.identifier.pmid","15904066"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1426"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0031-9007"],["dc.title","Nanoscale resolution in the focal plane of an optical microscope"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3125"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Applied Physics Letters"],["dc.bibliographiccitation.lastpage","3127"],["dc.bibliographiccitation.volume","82"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Blanca, C. M."],["dc.contributor.author","Dyba, Marcus"],["dc.contributor.author","Kastrup, Lars"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:45:00Z"],["dc.date.available","2017-09-07T11:45:00Z"],["dc.date.issued","2003"],["dc.description.abstract","We report subdiffraction resolution in far-field fluorescence microscopy through laser-diode-stimulated emission depletion of molecular markers. The diode-generated focal intensities lead to a resolution improvement by similar to45% in both lateral directions. Excitation and stimulated emission are performed with electronically synchronized diode pulses of 50-70 ps and 300-400 ps duration, respectively. The subdiffraction resolution is utilized to resolve neighboring individual molecules."],["dc.identifier.doi","10.1063/1.1571656"],["dc.identifier.gro","3144107"],["dc.identifier.isi","000182570000063"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1695"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0003-6951"],["dc.title","Laser-diode-stimulated emission depletion microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S123.2"],["dc.bibliographiccitation.issue","suppl 1"],["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.lastpage","S123"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Wagner, Eva"],["dc.contributor.author","Hebisch, Elke"],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2017-09-07T11:53:09Z"],["dc.date.available","2017-09-07T11:53:09Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1093/cvr/cvu098.101"],["dc.identifier.gro","3145042"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2734"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","Oxford University Press (OUP)"],["dc.relation.issn","0008-6363"],["dc.title","P677Superresolution microscopy reveals proliferative T-Tubule remodeling as general disease mechanism in early stages of heart failure development"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","275"],["dc.bibliographiccitation.journal","New Journal of Physics"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Fölling, Jonas"],["dc.contributor.author","Dyba, Marcus"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:54Z"],["dc.date.available","2017-09-07T11:49:54Z"],["dc.date.issued","2006"],["dc.description.abstract","We demonstrate the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by photoswitching ensembles of optically bistable organic molecular markers from a non-fluorescent to a fluorescent state and back. The photoswitching is accomplished by an isomerization reaction of a photochromic compound serving as a reversible energy acceptor of a fluorescent compound. The surpassing of the diffraction barrier with power levels of only a few hundred W cm(-2) of continuous wave irradiation is evidenced both in the effective point spread function and in the fluorescence images of test samples."],["dc.identifier.doi","10.1088/1367-2630/8/11/275"],["dc.identifier.gro","3143590"],["dc.identifier.isi","000242548900003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1120"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1367-2630"],["dc.title","Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","246"],["dc.bibliographiccitation.issue","5873"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","249"],["dc.bibliographiccitation.volume","320"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:45Z"],["dc.date.available","2017-09-07T11:48:45Z"],["dc.date.issued","2008"],["dc.description.abstract","We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons of cultured neurons were recorded with stimulated emission depletion (STED) microscopy in a 2.5-micrometer by 1.8-micrometer field of view. By reducing the cross-sectional area of the focal spot by about a factor of 18 below the diffraction limit (260 nanometers), STED allowed us to map and describe the vesicle mobility within the highly confined space of synaptic boutons. Although restricted within boutons, the vesicle movement was substantially faster in nonbouton areas, consistent with the observation that a sizable vesicle pool continuously transits through the axons. Our study demonstrates the emerging ability of optical microscopy to investigate intracellular physiological processes on the nanoscale in real time."],["dc.identifier.doi","10.1126/science.1154228"],["dc.identifier.gro","3143316"],["dc.identifier.isi","000254836700048"],["dc.identifier.pmid","18292304"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/817"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0036-8075"],["dc.title","Video-rate far-field optical nanoscopy dissects synaptic vesicle movement"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","L67"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","L69"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Wurm, Christian Andreas"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","We demonstrate two-color fluorescence microscopy with nanoscale spatial resolution by applying stimulated emission depletion on fluorophores differing in their absorption and emission spectra. Green- and red-emitting fluorophores are selectively excited and quenched using dedicated beam pairs. The stimulated emission depletion beams deliver a lateral resolution of < 30 nm and 65 nm for the green and the red color channel, respectively. The similar to 5 nm alignment accuracy of the two images establishes the precision with which differently labeled proteins are correlated in space. Colocalized nanoscopy is demonstrated with endosomal protein patterns and by resolving nanoclusters of a mitochondrial outer membrane protein, Tom20, in relation with the F(1)F(0)ATP synthase. The joint improvement of resolution and colocalization demonstrates the emerging potential of far-field fluorescence nanoscopy to study the spatial organization of macromolecules in cells."],["dc.identifier.doi","10.1529/biophysj.107.104497"],["dc.identifier.gro","3143514"],["dc.identifier.isi","000245164000003"],["dc.identifier.pmid","17307826"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1037"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","Two-color far-field fluorescence nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","675"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","684"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:20Z"],["dc.date.available","2017-09-07T11:45:20Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicles need to be mobile to reach their release sites during synaptic activity. We investigated vesicle mobility throughout the synaptic vesicle cycle using both conventional and subdiffraction-resolution stimulated emission depletion fluorescence microscopy. Vesicle tracking revealed that recently endocytosed synaptic vesicles are highly mobile for a substantial time period after endocytosis. They later undergo a maturation process and integrate into vesicle clusters where they exhibit little mobility. Despite the differences in mobility, both recently endocytosed and mature vesicles are exchanged between synapses. Electrical stimulation does not seem to affect the mobility of the two types of vesicles. After exocytosis, the vesicle material is mobile in the plasma membrane, although the movement appears to be somewhat limited. Increasing the proportion of fused vesicles (by stimulating exocytosis while simultaneously blocking endocytosis) leads to substantially higher mobility. We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement."],["dc.identifier.doi","10.1016/j.bpj.2010.04.054"],["dc.identifier.gro","3142885"],["dc.identifier.isi","000280182300042"],["dc.identifier.pmid","20643088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/338"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","High- and Low-Mobility Stages in the Synaptic Vesicle Cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","305a"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.volume","102"],["dc.contributor.author","Kohl, Tobias"],["dc.contributor.author","Parlitz, Ulrich"],["dc.contributor.author","Lauterbach, Marcel"],["dc.contributor.author","Minh Tuan, Hoang-Trong"],["dc.contributor.author","Williams, George S.B."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Jafri, M. Saleet"],["dc.contributor.author","Lederer, W.J."],["dc.contributor.author","Luther, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.contributor.author","Lehnart, Stephan E."],["dc.date.accessioned","2022-03-01T11:44:54Z"],["dc.date.available","2022-03-01T11:44:54Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1016/j.bpj.2011.11.1683"],["dc.identifier.pii","S0006349511030311"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103157"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0006-3495"],["dc.title","STED Nanoscopy of Cardiac RyR2 Clusters and Sub-Structure Analysis After Myocardial Infarction"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]