Perera, Ruwan K.

[["dc.bibliographiccitation.artnumber","6965"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Maier, Lars S."],["dc.contributor.author","Hasenfuß, Gerd"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2017-09-07T11:44:27Z"],["dc.date.available","2017-09-07T11:44:27Z"],["dc.date.issued","2015"],["dc.description.abstract","3',5'-cyclic adenosine monophosphate (cAMP) is an ubiquitous second messenger that regulates physiological functions by acting in distinct subcellular microdomains. Although several targeted cAMP biosensors are developed and used in single cells, it is unclear whether such biosensors can be successfully applied in vivo, especially in the context of disease. Here, we describe a transgenic mouse model expressing a targeted cAMP sensor and analyse microdomain-specific second messenger dynamics in the vicinity of the sarcoplasmic/endoplasmic reticulum calcium ATPase (SERCA). We demonstrate the bio-compatibility of this targeted sensor and its potential for real-time monitoring of compartmentalized cAMP signalling in adult cardiomyocytes isolated from a healthy mouse heart and from an in vivo cardiac disease model. In particular, we uncover the existence of a phos-phodiesterase-dependent receptor-microdomain communication, which is affected in hypertrophy, resulting in reduced beta-adrenergic receptor-cAMP signalling to SERCA."],["dc.identifier.doi","10.1038/ncomms7965"],["dc.identifier.gro","3141928"],["dc.identifier.isi","000353704700017"],["dc.identifier.pmid","25917898"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2634"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/103"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.issn","2041-1723"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG L. Maier (Experimentelle Kardiologie)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.title","In vivo model with targeted cAMP biosensor reveals changes in receptor-microdomain communication in cardiac disease"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1235"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1245"],["dc.bibliographiccitation.volume","114"],["dc.contributor.author","Götz, Konrad R."],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Kuhn, Michaela"],["dc.contributor.author","Gorelik, Julia"],["dc.contributor.author","Balligand, Jean-Luc"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2018-05-07T12:50:07Z"],["dc.date.available","2018-05-07T12:50:07Z"],["dc.date.issued","2014"],["dc.description.abstract","3',5'-Cyclic guanosine monophosphate (cGMP) is an important second messenger that regulates cardiac contractility and protects the heart from hypertrophy. However, because of the lack of real-time imaging techniques, specific subcellular mechanisms and spatiotemporal dynamics of cGMP in adult cardiomyocytes are not well understood."],["dc.identifier.doi","10.1161/CIRCRESAHA.114.302437"],["dc.identifier.pmid","24599804"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14629"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/20"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.1161/CIRCRESAHA.114.302437"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","1524-4571"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Transgenic mice for real-time visualization of cGMP in intact adult cardiomyocytes"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1304"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Circulation Research"],["dc.bibliographiccitation.lastpage","1311"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Sprenger, Julia U."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Hübscher, Daniela"],["dc.contributor.author","Lehnart, Stephan Elmar"],["dc.contributor.author","Abesser, Marco"],["dc.contributor.author","Schuh, Kai"],["dc.contributor.author","El-Armouche, Ali"],["dc.contributor.author","Nikolaev, Viacheslav O."],["dc.date.accessioned","2018-05-07T11:53:29Z"],["dc.date.available","2018-05-07T11:53:29Z"],["dc.date.issued","2015"],["dc.description.abstract","Cyclic nucleotides are second messengers that regulate cardiomyocyte function through compartmentalized signaling in discrete subcellular microdomains. However, the role of different microdomains and their changes in cardiac disease are not well understood."],["dc.identifier.doi","10.1161/CIRCRESAHA.116.306082"],["dc.identifier.pmid","25688144"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/14623"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/82"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A01: cAMP- und cGMP- Mikrodomänen bei Herzhypertrophie und Insuffizienz"],["dc.relation","SFB 1002 | A09: Lokale molekulare Nanodomänen-Regulation der kardialen Ryanodin-Rezeptor-Funktion"],["dc.relation.doi","10.1161/CIRCRESAHA.116.306082"],["dc.relation.eissn","1524-4571"],["dc.relation.issn","1524-4571"],["dc.relation.workinggroup","RG El-Armouche"],["dc.relation.workinggroup","RG Lehnart (Cellular Biophysics and Translational Cardiology Section)"],["dc.relation.workinggroup","RG Nikolaev (Cardiovascular Research Center)"],["dc.title","Microdomain switch of cGMP-regulated phosphodiesterases leads to ANP-induced augmentation of β-adrenoceptor-stimulated contractility in early cardiac hypertrophy"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Cardiovascular Research"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Perera, Ruwan K."],["dc.contributor.author","Steinbrecher, Julia H."],["dc.contributor.author","Lehnart, Stephan E."],["dc.contributor.author","Nikolaev, V. O."],["dc.date.accessioned","2018-11-07T09:37:39Z"],["dc.date.available","2018-11-07T09:37:39Z"],["dc.date.issued","2014"],["dc.identifier.doi","10.1093/cvr/cvu098.37"],["dc.identifier.isi","000343730100471"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32885"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.publisher.place","Oxford"],["dc.relation.eventlocation","Barcelona, SPAIN"],["dc.title","ANP potentiates catecholaminergic stress in early cardiac disease"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]