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MITRAC15/COA1 promotes mitochondrial translation in a ND2 ribosome-nascent chain complex
ISSN
1469-221X
1469-3178
Date Issued
2020-01-07
Author(s)
Wang, Cong
Pacheu-Grau, David
Liu, Fan
Zhu, Ying
Dennerlein, Sven
DOI
10.15252/embr.201948833
Abstract
The mitochondrial genome encodes for thirteen core subunits of the oxidative phosphorylation system. These proteins assemble with imported proteins in a modular manner into stoichiometric enzyme complexes. Assembly factors assist in these biogenesis processes by providing co-factors or stabilizing transient assembly stages. However, how expression of the mitochondrial-encoded subunits is regulated to match the availability of nuclear-encoded subunits is still unresolved. Here, we address the function of MITRAC15/COA1, a protein that participates in complex I biogenesis and complex IV biogenesis. Our analyses of a MITRAC15 knockout mutant reveal that MITRAC15 is required for translation of the mitochondrial-encoded complex I subunit ND2. We find that MITRAC15 is a constituent of a ribosome-nascent chain complex during ND2 translation. Chemical crosslinking analyses demonstrate that binding of the ND2-specific assembly factor ACAD9 to the ND2 polypeptide occurs at the C-terminus and thus downstream of MITRAC15. Our analyses demonstrate that expression of the founder subunit ND2 of complex I undergoes regulation. Moreover, a ribosome-nascent chain complex with MITRAC15 is at the heart of this process.
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Wang_et_al-2019-EMBO_reports.pdf
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2265898331690948c5483a3b403b304c