Molecular cytogenetic monitoring from CD34+peripheral blood cells in myelodysplastic syndromes: First results from a prospective multicenter German diagnostic study

ISSN
0145-2126
Date Issued
2013
Author(s)
Götze, Katharina S.
Müller-Thomas, Catharina
Platzbecker, Uwe
Germing, Ulrich
Brümmendorf, Tim H.
Bug, Gesine
Ottmann, Oliver G.
Giagounidis, Aristoteles A. N.
Stadler, Michael
Hofmann, Wolf-Karsten
Schafhausen, Philippe
Lübbert, Michael
Schlenk, Richard F.
Blau, Igor W.
Ganster, Christina
Metz, Michael
Detken, Sven
Seraphin, Jörg
Jentsch-Ullrich, Kathleen
Böhme, Angelika
DOI
10.1016/j.leukres.2013.03.019
Abstract
The gold standard of cytogenetic analysis in myelodysplastic syndromes (MDS) is conventional chromosome banding (CCB) analysis of bone marrow (BM) metaphases. Most aberrations can also be detected by fluorescence-in situ-hybridization (FISH). For this prospective multicenter German diagnostic study (www.clinicaltrials.gov: #NCT01355913) 360 patients, as yet, were followed up to 3 years by sequential FISH analyses of immunomagnetically enriched CD34+ peripheral blood (PB) cells using comprehensive FISH probe panels, resulting in a total number of 19,516 FISH analyses. We demonstrate that CD34+ PB FISH correlates significantly with CCB analysis and represents a feasible method for a reliable non-invasive cytogenetic monitoring from PB. (C) 2013 Elsevier Ltd. All rights reserved.