Grabe, Niels

[["dc.bibliographiccitation.firstpage","2037"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","International Journal of Cancer"],["dc.bibliographiccitation.lastpage","2046"],["dc.bibliographiccitation.volume","136"],["dc.contributor.author","Funk, Sonja"],["dc.contributor.author","Mark, Regina"],["dc.contributor.author","Bayo, Pilar"],["dc.contributor.author","Flechtenmacher, Christa"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Angel, Peter"],["dc.contributor.author","Plinkert, Peter K."],["dc.contributor.author","Hess, Jochen"],["dc.date.accessioned","2022-05-02T08:59:27Z"],["dc.date.available","2022-05-02T08:59:27Z"],["dc.date.issued","2015-05-01"],["dc.description.abstract","S100/calgranulins (S100A8, S100A9 and S100A12) are key players of innate immune function and elevated levels are a characteristic feature of acute and chronic inflammation, and inflammation-associated carcinogenesis. However, reduced S100A8 and S100A9 expression has been detected for squamous cell carcinoma, including the head and neck region (HNSCC), which originate from mucosal epithelia with abundant expression of both proteins under physiological conditions. In contrast to S100A8 and S100A9, only sparse information is available for S100A12 and a comparative study of all three S100/calgranulins in HNSCC is still missing. We analyzed S100/calgranulin protein levels in a retrospective patient cohort (n = 131) of oropharyngeal squamous cell carcinoma (OPSCC) by immunohistochemical staining of tissue microarrays. Common characteristics of all three S100/calgranulins were: (i) abundant expression in supra-basal keratinocytes of normal mucosa with predominant nuclear staining, (ii) low expression in 30.4-51.9% of primary OPSCCs and (iii) variable accumulation of S100/calgranulin-positive immune cells in the tumor stroma. These features were associated with histopathological characteristics, such as tumor grade, lymph node metastasis and tumor stage. Furthermore, univariate and multivariate analysis revealed worse overall survival of OPSCC patients with simultaneous reduction of S100A8 and S100A12 expression, while expression of S100A9 or presence of the S100A8/S100A9 heterodimer had no impact, suggesting distinct regulation and function of individual S100/calgranulins in the pathogenesis of HNSCCs."],["dc.identifier.doi","10.1002/ijc.29262"],["dc.identifier.pmid","25302747"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107538"],["dc.language.iso","en"],["dc.relation.eissn","1097-0215"],["dc.relation.issn","0020-7136"],["dc.title","High S100A8 and S100A12 protein expression is a favorable prognostic factor for survival of oropharyngeal squamous cell carcinoma"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","109"],["dc.bibliographiccitation.issue","1-2"],["dc.bibliographiccitation.journal","Cellular Oncology"],["dc.bibliographiccitation.lastpage","119"],["dc.bibliographiccitation.volume","32"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Pommerencke, Thora"],["dc.contributor.author","von Knebel Doeberitz, Magnus"],["dc.contributor.author","Reuschenbach, Miriam"],["dc.contributor.author","Wentzensen, Nicolas"],["dc.date.accessioned","2022-04-29T14:52:57Z"],["dc.date.available","2022-04-29T14:52:57Z"],["dc.date.issued","2010"],["dc.description.abstract","Although cytological screening for cervical precancers has led to a reduction of cervical cancer incidence worldwide it is a subjective and variable method with low single-test sensitivity. New biomarkers like p16 that specifically highlight abnormal cervical cells can improve cytology performance. Virtual microscopy offers an ideal platform for assisted evaluation and archiving of biomarker-stained slides."],["dc.identifier.doi","10.3233/CLO-2009-0508"],["dc.identifier.pmid","20208139"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107107"],["dc.language.iso","en"],["dc.relation.eissn","1875-8606"],["dc.title","A virtual microscopy system to scan, evaluate and archive biomarker enhanced cervical cytology slides"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1489"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1496"],["dc.bibliographiccitation.volume","36"],["dc.contributor.author","Schmoch, Thomas"],["dc.contributor.author","Gal, Zoltan"],["dc.contributor.author","Mock, Andreas"],["dc.contributor.author","Mossemann, Jan"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Schmezer, Peter"],["dc.contributor.author","Lasitschka, Felix"],["dc.contributor.author","Beckhove, Philipp"],["dc.contributor.author","Unterberg, Andreas"],["dc.contributor.author","Herold-Mende, Christel"],["dc.date.accessioned","2022-05-02T09:02:43Z"],["dc.date.available","2022-05-02T09:02:43Z"],["dc.date.issued","2016-04"],["dc.description.abstract","Recently, anti-tumourigenic effects of all-trans-retinoic-acid (ATRA) on glioblastoma stem cells were demonstrated. Therefore we investigated if these beneficial effects could be enhanced by co-medication with epigenetic drugs such as the histone deacetylase (HDAC) inhibitor suberoylanilide hydroxamic acid (SAHA) or the DNA-methyltransferase inhibitor 5-aza-2'deoxycytidine (5-AZA)."],["dc.identifier.pmid","27069124"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107543"],["dc.language.iso","en"],["dc.relation.eissn","1791-7530"],["dc.title","Combined Treatment of ATRA with Epigenetic Drugs Increases Aggressiveness of Glioma Xenografts"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","170"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Medicine"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Jungk, Christine"],["dc.contributor.author","Mock, Andreas"],["dc.contributor.author","Exner, Janina"],["dc.contributor.author","Geisenberger, Christoph"],["dc.contributor.author","Warta, Rolf"],["dc.contributor.author","Capper, David"],["dc.contributor.author","Abdollahi, Amir"],["dc.contributor.author","Friauf, Sara"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Beckhove, Philipp"],["dc.contributor.author","von Deimling, Andreas"],["dc.contributor.author","Unterberg, Andreas"],["dc.contributor.author","Herold-Mende, Christel"],["dc.date.accessioned","2022-04-29T14:54:16Z"],["dc.date.available","2022-04-29T14:54:16Z"],["dc.date.issued","2016"],["dc.description.abstract","The spatial relationship of glioblastoma (GBM) to the subventricular zone (SVZ) is associated with inferior patient survival. However, the underlying molecular phenotype is largely unknown. We interrogated an SVZ-dependent transcriptome and potential location-specific prognostic markers."],["dc.identifier.doi","10.1186/s12916-016-0710-7"],["dc.identifier.pmid","27782828"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107126"],["dc.language.iso","en"],["dc.relation.issn","1741-7015"],["dc.title","Spatial transcriptome analysis reveals Notch pathway-associated prognostic markers in IDH1 wild-type glioblastoma involving the subventricular zone"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4193"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","Annals of Surgical Oncology"],["dc.bibliographiccitation.lastpage","4201"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Kahlert, Christoph"],["dc.contributor.author","Gaitzsch, Eva"],["dc.contributor.author","Steinert, Gunnar"],["dc.contributor.author","Mogler, Carolin"],["dc.contributor.author","Herpel, Esther"],["dc.contributor.author","Hoffmeister, Michael"],["dc.contributor.author","Jansen, Lina"],["dc.contributor.author","Benner, Axel"],["dc.contributor.author","Brenner, Hermann"],["dc.contributor.author","Chang-Claude, Jenny"],["dc.contributor.author","Rahbari, Nuh"],["dc.contributor.author","Schmidt, Thomas"],["dc.contributor.author","Klupp, Fee"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Koch, Moritz"],["dc.contributor.author","Halama, Niels"],["dc.contributor.author","Büchler, Markus"],["dc.contributor.author","Weitz, Juergen"],["dc.date.accessioned","2022-04-29T14:53:50Z"],["dc.date.available","2022-04-29T14:53:50Z"],["dc.date.issued","2012-12"],["dc.description.abstract","Aldehyde dehydrogenase 1A1 (ALDH1A1) has been described as a cancer stem cell marker and as a regulator of cellular chemoresistance. Therefore, ALDH1A1 has been suggested as potential biomarker to stratify patients into different risk categories for a \"personalized\" therapy approach. We have investigated the prognostic role of ALDH1A1 in primary colorectal cancer and its value in predicting response to chemotherapy in metastatic colorectal cancer."],["dc.identifier.doi","10.1245/s10434-012-2518-9"],["dc.identifier.pmid","22878609"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107119"],["dc.language.iso","en"],["dc.relation.eissn","1534-4681"],["dc.relation.issn","1068-9265"],["dc.title","Expression analysis of aldehyde dehydrogenase 1A1 (ALDH1A1) in colon and rectal cancer in association with prognosis and response to chemotherapy"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e24116"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","OncoImmunology"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Halama, Niels"],["dc.contributor.author","Spille, Anna"],["dc.contributor.author","Lerchl, Tina"],["dc.contributor.author","Brand, Karsten"],["dc.contributor.author","Herpel, Esther"],["dc.contributor.author","Welte, Stefan"],["dc.contributor.author","Keim, Sophia"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Klupp, Fee"],["dc.contributor.author","Kahlert, Christoph"],["dc.contributor.author","Weitz, Jürgen"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Jaeger, Dirk"],["dc.contributor.author","Zoernig, Inka"],["dc.date.accessioned","2022-04-29T14:52:41Z"],["dc.date.available","2022-04-29T14:52:41Z"],["dc.date.issued","2013-04-01"],["dc.description.abstract","The immune system plays an important role in shaping the clinical course of colorectal cancer (CRC). However, it is still unclear how the immune infiltrates of primary CRC lesions and distant metastases by immune effector cells are related to each other. To address this issue, we quantified CD3+, CD8+ and granzyme B+ lymphocytes in primary CRC samples and corresponding liver metastases. This analysis showed that the prognostic predictions that can be drawn from the infiltration of immune cells in primary CRCs and their metastases are heterogeneous. To investigate whether such heterogeneity would also be observed within CRC hepatic metastases, the density of the immune infiltrate and cytokine production were assessed in opposite sides of the same metastatic lesion. In addition, tumor-infiltrating lymphocytes were assessed in sequential sections of the same metastatic lesion, with a spacing of 30 μm. In summary, consistent cell counts and cytokine levels were detected within the same lesion. The study of a case of synchronous metastases, however, suggested that different metastatic lesions within the same patient may be heterogeneous, perhaps indicating a major impact for local causes on tumor infiltration by immune cells. In summary, our study demonstrates a consistent degree of heterogeneity between primary tumors and hepatic metastases but an excellent intra-lesional homogeneity. These findings may be of key importance for patient stratification and the development of personalized strategies against CRC."],["dc.identifier.doi","10.4161/onci.24116"],["dc.identifier.pmid","23734335"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107103"],["dc.language.iso","en"],["dc.relation.issn","2162-4011"],["dc.title","Hepatic metastases of colorectal cancer are rather homogeneous but differ from primary lesions in terms of immune cell infiltration"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Tsingos, Erika"],["dc.contributor.author","Höckendorf, Burkhard"],["dc.contributor.author","Sütterlin, Thomas"],["dc.contributor.author","Kirchmaier, Stephan"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Centanin, Lazaro"],["dc.contributor.author","Wittbrodt, Joachim"],["dc.date.accessioned","2022-04-29T14:52:36Z"],["dc.date.available","2022-04-29T14:52:36Z"],["dc.date.issued","2019"],["dc.description.abstract","Combining clonal analysis with a computational agent based model, we investigate how tissue-specific stem cells for neural retina (NR) and retinal pigmented epithelium (RPE) of the teleost medaka (Oryzias latipes) coordinate their growth rates. NR cell division timing is less variable, consistent with an upstream role as growth inducer. RPE cells divide with greater variability, consistent with a downstream role responding to inductive signals. Strikingly, the arrangement of the retinal ciliary marginal zone niche results in a spatially biased random lineage loss, where stem- and progenitor cell domains emerge spontaneously. Further, our data indicate that NR cells orient division axes to regulate organ shape and retinal topology. We highlight an unappreciated mechanism for growth coordination, where one tissue integrates cues to synchronize growth of nearby tissues. This strategy may enable evolution to modulate cell proliferation parameters in one tissue to adapt whole-organ morphogenesis in a complex vertebrate organ."],["dc.identifier.doi","10.7554/eLife.42646"],["dc.identifier.pmid","30910010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107102"],["dc.language.iso","en"],["dc.relation.eissn","2050-084X"],["dc.title","Retinal stem cells modulate proliferative parameters to coordinate post-embryonic morphogenesis in the eye of fish"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","35"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","IEEE Transactions on Medical Imaging"],["dc.bibliographiccitation.lastpage","46"],["dc.bibliographiccitation.volume","37"],["dc.contributor.author","Yin, Yi"],["dc.contributor.author","Sedlaczek, Oliver"],["dc.contributor.author","Muller, Benedikt"],["dc.contributor.author","Warth, Arne"],["dc.contributor.author","Gonzalez-Vallinas, Margarita"],["dc.contributor.author","Lahrmann, Bernd"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Kauczor, Hans-Ulrich"],["dc.contributor.author","Breuhahn, Kai"],["dc.contributor.author","Vignon-Clementel, Irene E."],["dc.contributor.author","Drasdo, Dirk"],["dc.date.accessioned","2022-04-29T14:55:36Z"],["dc.date.available","2022-04-29T14:55:36Z"],["dc.date.issued","2018"],["dc.description.abstract","Diffusion-weighted magnetic resonance imaging (DWI) is a key non-invasive imaging technique for cancer diagnosis and tumor treatment assessment, reflecting Brownian movement of water molecules in tissues. Since densely packed cells restrict molecule mobility, tumor tissues produce usually higher signal (a.k.a. less attenuated signal) on isotropic maps compared with normal tissues. However, no general quantitative relation between DWI data and the cell density has been established. In order to link low-resolution clinical cross-sectional data with high-resolution histological information, we developed an image processing and analysis chain, which was used to study the correlation between the diffusion coefficient (D value) estimated from DWI and tumor cellularity from serial histological slides of a resected non-small cell lung cancer tumor. Color deconvolution followed by cell nuclei segmentation was performed on digitized histological images to determine local and cell-type specific 2d (two-dimensional) densities. From these, the 3d cell density was inferred by a model-based sampling technique, which is necessary for the calculation of local and global 3d tumor cell count. Next, DWI sequence information was overlaid with high-resolution CT data and the resected histology using prominent anatomical hallmarks for co-registration of histology tissue blocks and non-invasive imaging modalities' data. The integration of cell numbers information and DWI data derived from different tumor areas revealed a clear negative correlation between cell density and D value. Importantly, spatial tumor cell density can be calculated based on DWI data. In summary, our results demonstrate that tumor cell count and heterogeneity can be predicted from DWI data, which may open new opportunities for personalized diagnosis and therapy optimization."],["dc.identifier.doi","10.1109/TMI.2017.2698525"],["dc.identifier.pmid","28463188"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107146"],["dc.language.iso","en"],["dc.relation.eissn","1558-254X"],["dc.relation.issn","0278-0062"],["dc.title","Tumor Cell Load and Heterogeneity Estimation From Diffusion-Weighted MRI Calibrated With Histological Data: an Example From Lung Cancer"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1954"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.lastpage","1968"],["dc.bibliographiccitation.volume","135"],["dc.contributor.author","Waldera Lupa, Daniel M."],["dc.contributor.author","Kalfalah, Faiza"],["dc.contributor.author","Safferling, Kai"],["dc.contributor.author","Boukamp, Petra"],["dc.contributor.author","Poschmann, Gereon"],["dc.contributor.author","Volpi, Elena"],["dc.contributor.author","Götz-Rösch, Christine"],["dc.contributor.author","Bernerd, Francoise"],["dc.contributor.author","Haag, Laura"],["dc.contributor.author","Huebenthal, Ulrike"],["dc.contributor.author","Fritsche, Ellen"],["dc.contributor.author","Boege, Fritz"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Tigges, Julia"],["dc.contributor.author","Stühler, Kai"],["dc.contributor.author","Krutmann, Jean"],["dc.date.accessioned","2022-05-02T08:58:07Z"],["dc.date.available","2022-05-02T08:58:07Z"],["dc.date.issued","2015-08"],["dc.description.abstract","Most molecular hallmarks of cellular senescence have been identified in studies of cells aged in vitro by driving them into replicative or stress-induced senescence. Comparatively, less is known about the characteristic features of cells that have aged in vivo. Here we provide a systematic molecular analysis of normal human dermal fibroblasts (NHDFs) that were isolated from intrinsically aged human skin of young versus middle aged versus old donors. Intrinsically aged NHDFs in culture exhibited more frequently nuclear foci positive for p53 binding protein 1 and promyelocytic leukemia protein reminiscent of 'DNA segments with chromatin alterations reinforcing senescence (DNA-SCARS)'. Formation of such foci was neither accompanied by increased DNA double strand breaks, nor decreased cell viability, nor telomere shortening. However, it was associated with the development of a secretory phenotype, indicating incipient cell senescence. By quantitative analysis of the entire secretome present in conditioned cell culture supernatant, combined with a multiplex cytokine determination, we identified 998 proteins secreted by intrinsically aged NHDFs in culture. Seventy of these proteins exhibited an age-dependent secretion pattern and were accordingly denoted 'skin aging-associated secreted proteins (SAASP)'. Systematic comparison of SAASP with the classical senescence-associated secretory phenotype (SASP) revealed that matrix degradation as well as proinflammatory processes are common aspects of both conditions. However, secretion of 27 proteins involved in the biological processes of 'metabolism' and 'adherens junction interactions' was unique for NHDFs isolated from intrinsically aged skin. In conclusion, fibroblasts isolated from intrinsically aged skin exhibit some, but not all, molecular hallmarks of cellular senescence. Most importantly, they secrete a unique pattern of proteins that is distinct from the canonical SASP and might reflect specific processes of skin aging."],["dc.identifier.doi","10.1038/jid.2015.120"],["dc.identifier.pmid","25815425"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107519"],["dc.language.iso","en"],["dc.relation.eissn","1523-1747"],["dc.relation.issn","0022-202X"],["dc.title","Characterization of Skin Aging-Associated Secreted Proteins (SAASP) Produced by Dermal Fibroblasts Isolated from Intrinsically Aged Human Skin"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","107"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Cancer"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Schrader, Carola H"],["dc.contributor.author","Kolb, Markus"],["dc.contributor.author","Zaoui, Karim"],["dc.contributor.author","Flechtenmacher, Christa"],["dc.contributor.author","Grabe, Niels"],["dc.contributor.author","Weber, Klaus-Josef"],["dc.contributor.author","Hielscher, Thomas"],["dc.contributor.author","Plinkert, Peter K."],["dc.contributor.author","Hess, Jochen"],["dc.date.accessioned","2022-04-29T14:54:06Z"],["dc.date.available","2022-04-29T14:54:06Z"],["dc.date.issued","2015-05-20"],["dc.description.abstract","Dysregulated expression of Kallikrein-related peptidase 6 (KLK6) is a common feature for many human malignancies and numerous studies evaluated KLK6 as a promising biomarker for early diagnosis or unfavorable prognosis. However, the expression of KLK6 in carcinomas derived from mucosal epithelia, including head and neck squamous cell carcinoma (HNSCC), and its mode of action has not been addressed so far."],["dc.identifier.doi","10.1186/s12943-015-0381-6"],["dc.identifier.pmid","25990935"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/107123"],["dc.language.iso","en"],["dc.relation.issn","1476-4598"],["dc.title","Kallikrein-related peptidase 6 regulates epithelial-to-mesenchymal transition and serves as prognostic biomarker for head and neck squamous cell carcinoma patients"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]